Dr. Khaled Sabarneh and Professor Firas Al-Fahaili publish a study entitled Over-Expression, Purification, and Characterization of Plant
Title: Over-Expression, Purification, and Characterization of Plant
LeGST01, the Mammalian GST-Omega Homolog that Interacts
with LeTPX1 from a Tomato cDNA Library.
The study focuses on an in vivo GST- omega homologue (pCRT7/TPxII intB4) over-expression, purification and characterization.
Experiments purport to characterize the antioxidant activity of the LeTPx1, the interacting glutathione S-transferases
BI-GST/GPx, LeGST-T1, T2, T3, T4, T5 and the mammalian inhibitor of apoptosis Bcl-2. Upon specific
expression, the proteins exerted, differential protective effects in yeast cells treated with lethal doses of the prooxidants hydrogen
peroxide, t-butyl hydroperoxide, and cumene hydroperoxide. The antioxidant activity of LeTPx1 was highest against
the cumene hydroperoxide. The overexpressing GST (omega) homologue TPxintB4 (Baier and Dietz, 1999) which share a
considerable homology of the mammalian GST-omega1. In conclusion, the work shows that yeast parental strains are extremely
sensitive to very low concentrations (0.2mM) of Cumenehydroperoxide. However, after applying the different antioxidants;
it appears that the smallest concentrations t to be tolerated.